Scientist II Downstream Purification and Characterization
Company: Thermo Fisher Scientific Inc.
Posted on: June 9, 2021
The position is a member of the Cell Biology R&D team
responsible for identifying and developing novel technologies for
incorporation into new Thermo Fisher products, generating
application data and supporting marketing initiatives. The
individual will initiate, direct and execute scientific research
individually and as part of cross-functional development teams to
reach these goals.
The individual will work as part of the Cell Biology R&D
team to support on-going projects within the Thermo Fisher product
commercialization process under the Gibco brand with future growth
opportunity for independent project leadership. The successful
candidate will possess relevant protein
purification/characterization skills for antibody and non-antibody
recombinant proteins and an innovative mindset focused around these
areas. Strong organizational skills will be necessary to support
protein characterization needs for multiple projects. Ability to
multitask and maintain a results-oriented focus is essential.
The successful candidate will have strong expertise in the
purification and characterization of proteins expressed in
mammalian and insect transient expression systems. Experience in
viral vector downstream process development (specifically AAV and
LV) and characterization is a plus. The candidate will have a
strong background in protein purification by column chromatography
(Affinity, IEX, NiNTA, reverse-phase, etc), depth filtration,
tangential flow filtration (TFF), and related approaches. The
candidate will also have the ability to characterize proteins to
determine concentration, purity, aggregation, fragmentation, glycan
profiles, charge variants and biological activity. Knowledge of
mass spectrometry-based proteomics is a plus.
The candidate will be required to maintain accurate lab
notebooks and training files in accordance with relevant Thermo
Fisher required standards to support patent application and defense
as well as other international standards (e.g., ISO compliance,
GLP/GMP requirements, etc.).
Typical requirements will include preparing and presenting
experimental data summaries and protocol writing. Perform
literature searches related to products under development. Work
with customers to provide R&D support on technical issues.
Interact with scientists in other business units to troubleshoot
problems and identify new opportunities. The successful candidate
will make contributions to scientific literature and conferences
and comply with all company safety regulations and procedures.
Strong background in protein purification/characterization with
a Bachelor's degree and 3+ years of relevant academic or industry
experience, a Master's degree plus 1-3+ years of experience.
- Purification of intracellular, and secreted proteins from
mammalian and insect cells
- Protein purification and analytical characterization by
standard laboratory techniques including AKTA-FPLC, SDS-PAGE,
Western Blot, SEC-HPLC, IEX-HPLC, RP-HPLC, HIC, HILIC, affinity
chromatography, ELISA, ligand-binding assays, functional cell based
- Viral vector purification and characterization is a plus.
- Knowledge of Mass Spectrometry-based proteomics is a plus.
- Established experience with technology transfer, particularly
- Demonstrated ability to creatively utilize existing analytical
technologies for new applications
- Troubleshoot and problem solve instrumentation hardware and
software related issues for FPLC and HPLCs (e.g. AKTA Pure FPLC,
AKTA Avant FPLC and Vanquish UHPLC)
- Provides customer applications support for existing
- Demonstrated track record of completing tasks within
established time lines
- Solid technical writing skills
- Excellent time management skills and multi-tasking
- Excellent organizational skills
- Strong verbal and written communication skills and the ability
to interpret, summarize, and present scientific results in a clear,
concise, accurate manner to both technical and non-technical
- Demonstrated ability to generate new ideas, achieve results and
Works in a laboratory environment. May be required to stand for
long periods of time while performing duties. Must be able to work
safely with chemicals and hazardous materials.
Keywords: Thermo Fisher Scientific Inc., Frederick , Scientist II Downstream Purification and Characterization, Other , Frederick, Maryland
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