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Post Doctoral Fellow

Company: Frederick National Laboratory for Cancer Research
Location: Frederick
Posted on: December 28, 2019

Job Description:

LHRI - The Laboratory of Human Retrovirology and Immunoinformatics (LHRI), Clinical Services Program, Applied/ Developmental Directorate, has investigated HIV pathogenesis and innate immune response against HIV or HIV-related virus infection. LHRI has several single nucleotide polymorphisms (SNPs) in HIV genome from patients infected with multiple drug resistant (MDR) HIV strains. In this position, a candidate will investigate and characterize the impact of the SNPs in drug sensitivity and viral fitness collaborating with National Institute of Allergy and Infectious Diseases (NIAID). KEY ROLES/RESPONSIBILITIES Development of a novel gene therapies to suppress MDR-HIV or other virus infection utilizing a combination of CRISPR/Case9 (or other gene editing methods) and other protocols BASIC QUALIFICATIONS To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below: Possession of a PhD or MD/PhD degree from an accredited college or university according to the Council for Higher Education Accreditation. Foreign degrees must be evaluated for U.S. equivalency No experience beyond the Doctorate degree required Must be able to obtain and maintain a security clearance PREFERRED QUALIFICATIONS Candidates with these desired skills will be given preferential consideration: General molecular biology techniques, construction of expression plasmids Tissue culture (cell lines and primary human cells) DNA transfection to primary cells using 4D system PCR, qRT-PCR Western blotting Immune precipitation assay Protein isolation using FPLC Imaging analysis of a Confocal or fluorescence microscopy CRISPR technology Handling of replication competent HIV virus and lentivirus AMNIS EXPECTED COMPETENCIES Strong background in immunology and knowledge in gene editing, CRISPR or other techniques The ability to challenge and establish new assay system(s) Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)

Keywords: Frederick National Laboratory for Cancer Research, Frederick , Post Doctoral Fellow, Other , Frederick, Maryland

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